The first floor public areas and part of the ground floor program space in the Museum are accessible to visitors in wheelchairs.

The main entrance to the Museum is located on 2nd Street with the lift accessible entrance located to the side of the Museum next to the Hart Senate Office Building. There is an exterior lift as well as an interior lift to provide access to the main exhibitions.  An accessible entrance is located on the main level. The doors facing Constitution Avenue are not entrances.